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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, happen to be explained. Two extra splice variants had been noted but thorough nucleotide sequence information and facts is not but readily available.

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Blocking peptides are peptides that bind especially to your goal antibody and block antibody binding. These peptide ordinarily includes the epitope regarded by the antibody.

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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible success are...

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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to make sure the absolute best benefits are...

Antibodies certain to the blocking peptide no more bind on the epitope about the goal protein. By evaluating the staining with the blocked antibody versus the antibody by itself, you can see which staining is precise.

Focus on Information and facts Cyclin E belongs to the remarkably conserved cyclin household, whose members are characterized by a spectacular periodicity in protein abundance in the cell cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show distinctive expression and degradation styles which contribute to the temporal coordination of each mitotic event. Cyclin E types a complex with and capabilities as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates with the G1-S stage boundary and it is degraded as cells progress by S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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